Department of Medical Microbiology


The Department of Medical Microbiology shall be at the forefront of scientific advances and technological developments in public health and medical microbiology. It shall be the Center of Excellence in microbiology education, training and research.


The Department of Medical Microbiology shall constantly provide the highest quality of advanced instruction, professional education, training, basic and applied research and community service and thereby produce outstanding scholars, practitioners and leaders in public health and medical microbiology.

Degree Progams

Doctor of Public Health
Master of Science in Public Health (Medical Microbiogy)

Training Programs

  1. Postgraduate Course in Diagnostic Mycology
  2. Food and Water Bacteriology

Extension Services

  1. Acid-Fast Staining and Quantitative Microscopy (i.e. Sputum smears, Slit skin smears)
  2. Bacterial Isolation and Identification (Environmental, Food and Water, Products etc.)
  3. Bacteriological Water Analysis
  4. Fungal Culture and Isolation from Clinical Specimen
  5. Microscopic Agglutination Test for Leptospirosis Diagnosis
  6. Other Services (e.g. Bacterial Challenge, Kirby-Bauer AST, Microbial Researches etc)

Ongoing Researches/projects

  • Evaluation of Diagnostic Tests for Leptospirosis in the Philippines.
  • Quantitative Molecular Signatures & Predictors of Leptospirosis & the Development of its Complications Using Gene Expression Markers & Pathway Analyses
  • Prevalence Survey for Animal leptospirosis in Iloilo province


  • Development of Mini-ELISA for the Determination of Clinically Important Antigens and Antibodies
  • Seroepidemiology of HAV infection in Metro Manila
  • Development of Serotyping Reagents for the Detection of Cholera Species HIV-1 Tat Gene-based Edible Vaccine in Tomatoes
  • Effect of Psidium guajava leaf decoction as a mouthwash in normal subjects compared to Chlorhexidine 0.12% mouthwash and normal saline solution
  • A Comparative Study on the Mycobacterial effect of Using 5% Sodium Hypochlorite and 2% Glutaraldehyde in Sputum Processing for Acid Fast Bacili Detection
  • In-Vitro and In-Vivo studies on the Anti-TB Properties of Aistonia Scholaris (Dita), Alphonseae Arboraea(Anonaceae), Diclodiscus Paniculatus (Tillaceae) and Pisonia Umbrelifera (Nyctaginaceae)
  • Identification of the prevalent Leptospira from environmental samples in urban and rural areas of the Philippines
  • A Phase III, Double-blind Randomized Group Study to Compare the Protective Immune Response to Older Adults after Immunization with either Engerix-B HBV Vaccine of Recombinant HBV Surface Antigen Co-administered with Dynavax Immunostimulatory Phosphotionat
  • In-vitro Screening for the Anti-Dengue Activity of Vitex negundo (Lagundi)
  • Prevalence Survey for Leptospirosis Among Animals in Iloilo Province
  • A Phase IV, Single blind randomized, multicentric study
  • In-vitro Screening for Anti-TB Activity of Psidium guajav(guava)and Anona muricata (guyabano)
  • A Preliminary study on the effects of Mentha cordifolia Opiz. (Yerba Buena) on the NF-kappa B signaling pathway
  • A Study on the Activity of Crude and Protein Extracts of Medicinal Plants on HIV-1 Replication in Latently-infected cells
  • Isolation and Identification of Shiga-toxin E. coli(STEC) in feed samples from the Philippines
  • Evaluation of diagnostic tests for Leptospirosis in the Philippines
  • A Study on Emerging Foodborne Bacterial Pathogens in the Philippines
  • Prevalence of Yersinia enterocolitica in some Philippine Foods
  • Quantitative Molecular Signatures and Predictors of Leptospirosis and the development of its complications using gene expression markers and pathway analysis
  • Assessment of the Relevance of the BSPH Curriculum
  • Public Health Aspect of the Environmental Impact Assessment of the Coal-Fired Thermal Power Plant in Pinamucan, Batangas, Philippines
  • Development of Monovalent and Bivalent Leptospira Vaccines Applicable in the Philippine Setting
  • Characterization and Determination of the Cytokine and Macrophage Stimulating Activities of the Polysaccharide Fractions of Momordia charantia (Ampalaya)
  • Public Health and Epidemiological Research Needs and Issues Regarding Coconut Oil and Cardiovascular Diseases
  • Clinical Trial of Auranofin gold Compound in HIV-1 Infected Filipinos
  • Risk Assessment of Genetically Modified Organism (GMOs) and Potentially Harmful Exotic Species (PHES) Related to Agriculture and Fishery
  • Development of Alamar Blue-based Test an Alternate Method in Human Proliferation Assay
  • Salmonella typhi Flagellar Antigen Edible Vaccine

Publications/Creative Works

  • Masuzawa T, Sakakibara K, Saito M, Hidaka Y, Villanueva SYAM, Yanagihara Y, Yoshida S (2018). Characterization of Leptospira species isolated from soil collected in Japan. Microbiology and Immunology. (2018); 62 (1); 55-59
  • Sumalapao DEP, Janairo JIB and Gloriani NG. Dipole Moment, Solvation Energy, and Ovality Account for the Variations in the Biological Activity of HIV-1 Reverse Transcriptase Inhibitor Fragments. Annual Research & Review in Biology. (2018); 22(5); 1-8
  • Sumalapao DEP, Cabrera EC, Flores MJC, Amalin DM, Villarante NR, Altura MT and Glo-riani NG. Viability Kinetic Profile, Morphological Structure, and Physicochemical Characterization of Candida albicans Biofilm on Latex Silicone Surfaces. Annual Research & Review in Biology. (2018); 24 (3); 1-8
  • Lopez SM, Ramiro VR, and Roxas EA. Measuring Stigma and Discrimination towards People Living with HIV among Health Care Workers in a Tertiary, Government Teaching Hospital in the Philippines. Acta Medica Philippina. (2017); 51 (4); 319-326
  • Sarol LC, Lota MML, Dato MC, Medina RJ.. Rapid Assessment of AMS-Related Practices in Hospitals of Various Capacity Levels in the Philippines.. WHO-WPRO. (Technical Report). (2016); ;
  • Pinlac PAV, Castillo EC, Guevarra JP, Escartin IC, Caluag MEI, Granada CN, Tagunicar LB, Banda AD, Go JJJL, Kim JK, Sy CAS, Maceda AA, Gloriani NG. . The Status of NonCommunicableDisease Prevention and Control in the Philippines: A Systematic Review. Acta Medica Philippina. (2015); ;
  • Saito M, Villanueva SY, Masuzawa T, Haraguchi Y, Ita S, Miyahara S, Ozuru R, Yamaguchi T, Yoshimura M, Ikejiri M, Aramaki N, Amran MY, Muslich LT, Iida K, Yanagihara Y, Gloriani NG, Yoshida S.. The usefulness of semi-soild medium in the isolation of highly virulent Leptospira strains from wild rats in an urban area of Fukuoka, Japan. Doi:10.1111/1348-0421.12260.PMID:25890990. Microbiol. Immunol. (2015); 59(6); 322-30
  • Bungay AAC.. Sarcocystis spp. Parasitology in the Philippines (formerly Philippine Textbook of Medical Parasitology).. 3 rd edition.UP Press (Chapter in a Book). (2015); ;
  • Salvana EMT, Roxas EA, Penamora MG. Immunocompromised Hosts and Parasitic Infections. Chapter 8: Special Topics in Parasitology.In Vicente Y. Belizario Jr. and Winifreda U de Leon (eds). Medical Parasitology in the Philippines.. University of the Philippines Press 2015. (Chapter in a Book). (2015); ;
  • Gloriani NG, Cavinta LC, Roxas EA, Villanueva SYAM. Prevention and Control of Leptospirosis in the Philippines: A Manual for Health Workers.. DOST-PCHRD. (2015); ;
  • Takaya S, Nomura KH, Villanueva SYAM, Saito M, Nomura K, Gloriani NG, Yoshida S.. Identification of leptospiral 3-hydroxyacylCoA dehydrogenase released in the urine of infected hamsters.. BMC Microbiology 2014. (2014); 14; 132
  • Villanueva SYAM, Saito M, Baterna RA, Estrada CAM, Rivera AKB, Dato MC, Zamora PRF, Segawa T, Cavinta LL, Fukui T, Masuzawa T, Yanagihara Y, Gloriani NG, Yoshida S. . Leptospirarat-human relationship in Luzon, Philippines. Microbes and Infection xx . 1e9 (2014); ;
  • Sia Su G, Fernandez EL, Marasigan MM, Sia Su ML, Sison, M and Bungay, AA.. Isolation and colonial morphological characterization of fungal species in copper laden sediments of Calancan Bay, Marinduque. Annual Research and Review in Biology. (2014); 4 (11); 1777-1783
  • Villanueva SYAM, Saito M, Tsutsumi Y, Segawa T, Baterna RA, Chakraborty A, Asoh T, Miyahara S, Yanagihara Y, Cavinta LL, Gloriani NG, Yoshida S. . High virulence in hamsters of four dominant Leptospira serovars isolated from rats in the Philippines. Microbiology (2014). (2014); 160; 418–428
  • Lota MMM, Sison MAC, Latorre AAE.. A Retrospective Study on Extended Spectrum BetaLactamase Bacteria in the Philippines from 1999 – 2013. Acta Medica Philippina. (2014); 48 (1); 28-34
  • Satoshi M, Mitsumasa S, Takaaki K, Villanueva SYAMV, Gloriani NG and Yoshida S.. Destruction of the hepatocyte junction by intercellular invasion of Leptospira causes jaundice in a hamster model of Weil’s disease.. Int. J. Exp. Path. (2014). (2014); 95; 271-281
  • Tatsuma A, Mitsumasa S, Villanueva SYAM, Takaaki K, Gloriani NG, and Yoshida S.. Natural defense by saliva and mucosa against oral infection by Leptospira.Can. J. Microbiol.. . (2014); ;
  • Bungay AAC.. Guidelines on Animal Care and Use in Research and Instruction,. (IACUC Committee Members as authors, UP-NIH. (2013); ;
  • Mendoza M, Roxas E, et al.. Clinical Profile of Patients Diagnosed with Leptospirosis After a Typhoon: A Multicenter Study.. Southeast Asian J Trop Med Public Health . (2013). (2013); 44 (6); 1021-1035
  • Ortega, AR. Training Module on the Culture and Drug Susceptibility Tests for Mycobacterium Tuberculosis , project of National Tuberculosis Research Laboratory. RITM-DOH. (2013); ;
  • Saito M, Villanueva SYAM, Kawamura Y, Iida K, Tomida J, Kanemaru T, Kohno E, Miyahara S, Umeda A, Amako K, Gloriani NG and Yoshida S.. Leptospira idonii sp, nov. isolated from environmental water.. International J of Systematic and Evolutionary Microbiology (2013). (2013); 63; 2457-2462
  • Widiyanti D, Koizumi N, Fukui T, Muslich LT, Segawa T, Villanueva SYAM, Saito, M, Masuzawa T, Gloriani NG and Yoshida S.. Development of Immunochormatography-Based Methods for Detection of Leptospiral LPS Antigen in Urine.. . (2013); 20 (5); 6836
  • Arbiol J, Borja MP, Yabe M, Nomura H, Gloriani NG and Yoshida S.. Valuing Human Leptospirosis Prevention Using the Opportunity Cost of Labor.. International J of Environmental Research and Public Health.. (2013); 10; 1845-1860
  • Chakraborty A, Miyahara S, Segawa T, Tatsuma A, Mitsumasa S, Villanueva SYAM, Antara A, Ozuru R, Gloriani NG, Yanagihara Y, Shin-ichi Y.. Comparative Analysis of Leptospira Strains Isolated from Environmental Soil and Water in the Philippines and Japan.. Appl. Environ. Microbiol.2013. (2013); 79 (2); 601
  • Chen Y., Konieczka JH, Springer DJ, Bowen SE, Zhang J, Silao FGS, Bungay AAC, Bigol, UG. Nicolas, MG et. al. . Convergent evolution of Calcineurin pathway roles in thermotolerance and virulence in Candida glabrata. Genes, genomes and genetics . Genes, genomes and genetics. (2012); 2; 675-691
  • Gloriani N.G.,Gatchalian S, Hoet B, Bock HL. Comparison of two different formulations of a hepatitis B vaccination in Filipino Adults . Philippine Journal of Pediatrics. (2008); ;
  • Gloriani N.G.,Gatchalian S, Hoet B, Bock HL.. Comparison of two different formulations of a hepatitis B vaccination in Filipino Adults. Philippine Journal of Pediatrics.. (2008); ;
  • Kreth HW, Lee BW, Kosuwon P, Salazar J, Gloriani, N.G., Bock HL, Meurice F. Biodrugs.. Varilix: 16 years of global-experience with the first refrigerator - stable varicella vaccine.. . (2008); ;
  • Gloriani, N.G.. Straight Talk on Biotechnology. Chapter on Food Safety.. Ateneo Press Ed:B.D. Peczon and A.M. Manal... (2008); ;
  • Gloriani, N.G. and Yasutake Yanagihara.. Philippine Rats: Ecology and Management Chapter on Rodent - borne infections in the Philippine: a review of investigations and case reports on leptospirosis and hantavirus infection. . (2008); ; 195-204
  • Mendoza, B.,Chan, V., Gloriani, N.G.. Detection of Anti-HBs, Anti-HBc, and HbsAG among Pedriatric Vaccines Years after Primary Hepatitis B Immunization.. Philippine Journal of Science.. (2007); 136 (1); 57-63
  • Cavinta, L., Ramos S. and Gloriani,N.G.. Development of an Alamar blue-based test as an alternate method in human lymphocite proliferation assays.. Acta Medica Philippina.. (2007); 41 (2); 25-29
  • Mendoza, B.,Chan, V., Gloriani, N.G.. Detection of Anti-HBs, Anti-HBc, and HbsAG among Pedriatric Vaccines Years after Primary Hepatitis B Immunization. Philippine Journal of Science. (2007); 136 (1); 57-63
  • Gloriani, N.G.. Statistical Definition of Biotechnology: Identifying Philippine Concerns - Asian Perspective. Asian Biotechnology, Innovation and Development Issues in Measurement and Collection of Statistics.. . (2007); ; 31-35
  • Gloriani, NG.. Plant made pharmaceuticals (PMPs): Rabies Edible Vaccine Cusisine for the Community. Proceedings of the Rabies Forum Bridging the Gaps: Parenteral vs Oral Rabies Vaccine.. . (2007); ;
  • Sarol LC. Victoriano A and Gloriani, N.G.. PCR Patterns of the Flagellin typhii.. Acta Medica Philippina.. (2006); 40 (1); 37-41
  • Bungay, AA.. Food Technology and Globalization; Chapter on Risk Assestment in Food Safety and Technology. . (2006); ;
  • Cavinta LL, Gloriani, NG, Ramos SR and Regalia RS. Studies on the Immunomodulelating activities of the Polysaccharide Extracts from Momordica Charantia (Ampalaya).. Philippine Journal of Nutrition.. (2004); 50 (1-2); 10-27
  • Panter, de las Llagas, Tobias. The entomopathogenic fungus Culicinomyces as a biocontrol agent for mosquitoes in Microbial Biotechnology for Sustainable Development and Productivity. Rajak R.C. (ed). . R.C. (ed). Jodhpur, India : Scientific Publishers.. (1999); ; 422-433
  • Alday, Dancel, Tobias. Itraconazole in the Treatment of Superficial Mycoses in Filipinos.. Phil. Journal of Internal Medicine.. (1988); 27 (3); 179-187
  • Chan, Madraso, Bagoyo, Delino, Tobias. Bacteriological Analysis of Drinking Water in Bagong Ilog, Pasig, Caloocan, Capri, Novaliches and Marulas, Valenzuela from December 1981 to March 1986.. Health Monitoring Component of the MWSS Sanitation Project.. (1987); ;
  • Iwasaki H, Chagan-Yasutan H, Lenao PSA, Koizumi N, Nakajima C, Taurustiati D, Firmanto H, Lacuesta TL, Ashino Y, Suzuki Y, Gloriani NG, Telan FRO, Hattori T. . Combined antibody and DNA detection for early diagnosis of leptospirosis after a disaster.. Diagnostic Microbiology and Infectious Disease. ; ;
  • Sia Su G, David PD,Tan LA, Sison, MA, Ragraio, E, Arollado, E, De Guzman, T.. Phytochemical Screening and antimicrobial activity of Casicum frutescens Linn, Crude fruit extract on selected microorganisms.. J Pharm Biomed Sci.. ; 37; 1922-1926
  • Roman D, Salvana E, Penamora M, Roxas E, Leyritana K, and Saniel M.. Invasive Trichosporonosis in an AIDS Patient: Case Report and Review of the Literature.. International Journal Of STD and AIDS. July 2013.. ; ;
  • Roman D, Salvana E, Penamora M, Roxas E, Leyritana K, and Saniel M.. Trichosponosis in an AIDS Patient: Case Report and Review of the Literature. International Journal of STD and AIDS. ; ;
  • Ozuru R, Saito M, Kanemaru T, Miyahara S, Villanueva SYAM, Murray GL, Adler B, Fujii J, Yoshida S. Adipose tissue is the first colonization site of Leptospira interrogans in subcutaneously infected hamsters. PLOS One. ; ;
  • Sia Su,G, Duenas,K, Roderno,K, Sison, MA, Sia Su, ML, Ragrario,E, De Guzman, T, Heralde, F.. Distribution and Diversity of Marine Fungi in Manila Bay. 2014. Malaysian Journal of Microbiology (Scopus). . ; ;
  • Saito M, Miyahara S, Villanueva SYAM, Aramaki N, Ikejiri M, Kobayashi Y, Guevarra JP, Masuzawa T, Gloriani NG, Yanagihara Y and Yoshida S.. PCR and Culture Identification of Pathogenic Leptospira spp. from Coastal Soil in Leyte, Philippines, after a Storm Surge during Super Typhoon Haiyan (Yolanda). Appl. Environ. Microbiol. ; 80 (22); 6926
  • Tacastacas, Bacoro, Berba, Tobias-Altura, Sison, dela Cruz-Roa.. A 47 Year Old Male with Multiple Ulcerated Skin Plaques: A Case Report on Disseminated Histoplasmosis and a Review of Eight Previously Reported Cases of Histoplasmosis in the Philippines.. Philippine Journal of Microbiology & Infectious Diseases. ; 35 (1&2);
  • Villanueva SYAM, Baterna RA, Cavinta LL, Yanagihara Y, Gloriani NG, Yoshida S. Seroprevalence of leptospirosis among water buffaloes, pigs, and dogs in selected areas in the Philippines, 2007 to 2008. Acta Medica Philippina (in press). ; ;
  • Zhang J, Silao FGS, Bigol UJ, Bungay AAC, Nicolas MG, et al.. Calcineurin is required for pseudohyphal growth, virulence and resistance in Candida luisitaniae.. PLoS One (2012). ; 7 (8);
  • Tabo N, Villanueva SYAM, Gloriani N. Prevalence of Leptospira-agglutinating Antibodies in Abattoir workers and slaughtered an-imals in selected slaughterhouses in Cavite, Philippines. Philippine Journal of Science (in press). ; ;
  • Chen Y, Yu S, Huang H, Chang Y, Lehman VN, Silao FGS, Bigol UG, Bungay AAC, Averette A and Heitman J.. Calcineurin controls hyphal growth, virulence and drug tolerance of Candida tropicalis.. Eukaryotic EC (ASM). ; ;